We’ve previously used spheroid size as a measure of cell growth under different conditions, but this doesn’t account for cell density so simply isn’t as accurate. The W8 is the first machine that allows us to properly assess how our culture conditions, drugs or different cell lines really impact on the growth of our ‘mini tumours’. The W8 is so much quicker and easier to use than our previous approach of manually sizing our spheroids, and we can even sort them for downstream analysis. We think the W8 is going to be a game-changer for our brain cancer research projects
We have generated organoids from different GEMMs of prostate cancer and observed a plethora of different structures in the cultures system. The W8 has allowed us to associated their density and weights. We have then correlated these physical parameters with different status of aggressiveness of the caner. The W8 is precise instrumentation that will certainly change how we look into new parameters that will allow us to better define the cancer.
Being focused on innovative anti-tumor treatments, with a specific interest in 3D cellular models, some of my goals are related to sample standardization and to the culture of homogenous samples, which are needed to obtain the maximum reliability of the applied treatments. For this reason, I adopt multidisciplinary approaches to grow and characterize 3D spheroids.
In this context, I believe the W8 perfectly fits my research purposes, as it is the only instrument capable to perform the Biophysical analysis, which appeared to be a crucial characterization during the 3D cell models’ standardization. We noted how such biophysical results are giving a novel point to view, not only to highlight sample uniformity, but also to be correlated with the obtained biological results
I have been collaborating with CellDynamics since 2017 and have seen the technological level of the group grow exponentially. I believe CellDynamics to be one the most brilliant StartUp and an excellent scientific partner for the development of new investigation techniques on organoids and spheroids.
In recent years we have optimized a complete biophysical analysis of the spheroids that starts from the mass density data obtained with the W8, followed by the sorting of the subpopulations, and then to the clarification of the samples and finally to the microscopic analysis in deep imaging. This workflow allows a precise measurement of the spheroid characteristics and represent a key step forward for the accurate testing of treatment’s potential in 3D in vitro models
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