Tumor microenvironment (TME) has received attention as one of the most important responsible of tumor cell behavior and disease progression. In this application note, the creation of heterotypic spheroids composed of lung tumor cells and fibroblasts cells allow to live image the tumor-stroma interactions in 3D and in dynamics with CELLviewer.

The 3D multicellular spheroids offer an exciting model to investigate in vivo-like functionality where cells are grown in physiologically relevant conditions compared to monolayer 2D cell culture. Nevertheless, its wide applications are currently limited due to lack of reliable, reproducible and automated methods. The Sphericalplate 5D® is an integrated solution in CELLviewer workflow, delivering homogeneous and size-controlled spheroid populations, that tightly fit with the downstream CELLviewer analysis. These two technologies, combined together, represent streamlined and automated 3D cell culture method with enhanced test reproducibility and reliability.