16 luglio 2018

CELLviewer presentation

  CELLviewer raccontato da Daniele Gazzola (presidente di CellDynamics) e da Simone Pasqua (Founder e Product Menager di CellDynamics) con l’ intervento del Dr. Parmegiani (IVF Laboratory Director presso GynePro Medical Centers).

5 giugno 2018

In vitro generation of human pluripotent stem cell derived lung organoids

Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, Dye BR and colleagues from Department of Cell and Developmental Biology (University of Michigan Medical School) expose the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and […]

13 aprile 2018

Microgravity as a biological tool to examine host–pathogen interactions and to guide development of therapeutics

Space exploration programs have long been interested in the effects of spaceflight on biology. Bacterial behavior has been shown to be altered both in space and under simulated microgravity conditions. In some cases, bacteria appear attenuated, whereas in others virulence is enhanced. The microgravity environment can be exploited as a tool in the search for […]

6 aprile 2018

Scaffold-free tissue formation under real and simulated Microgravity

Scaffold-free tissue formation in microgravity is a new method in regenerative medicine and an important topic in Space Medicine. As spaceflights are rare and extremely expensive, cell culture under simulated microgravity allows more comprehensive and frequent studies on the scaffold-free 3D tissue formation in some aspects, as a number of publications have proven during the […]

29 marzo 2018

Microfluidic assays for mobile tumor cells in human body fluids: rethinking liquid biopsy

Liquid biopsy is a blood test involving the harvesting of tumor materials from peripheral blood. Tumor cells from non-blood body fluids have always been clinically available in cytological examinations but limited for use in differential diagnosis due to the low sensitivity of conventional cytopathology. With the recent significant progress in microfluidic and downstream molecular technologies, […]

21 marzo 2018

Diverse subtypes of CTCs with cytogenetic abnormalities in hepatocellular carcinoma patients determine cancer relapse

Detection of hepatocellular carcinoma (HCC) circulating tumor cells (CTCs) performed with conventional strategies, is significantly limited due to inherently heterogeneous and dynamic expression of EpCAM, as well as degradation of cytokeratins during epithelial-to-mesenchymal transition, which inevitably lead to non-negligible false negative detection of such “uncapturable and invisible” CTCs. A novel SE-iFISH (SE, subtraction enrichment; iFISH, […]

15 marzo 2018

Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform

Studies that rely on fluorescence imaging of nonadherent cells that are cultured in suspension, such as E. coli, are often hampered by trade-offs that must be made between data throughput and imaging resolution. A platform for microfluidics-assisted cell screening (MACS) was developed by key scientists from Department of Systems Biology Harvard Medical School (Boston, Massachusetts, […]

8 marzo 2018

Elasticity-based development of functionally enhanced multicellular 3D liver encapsulated in hybrid hydrogel

Current in vitro liver models provide 3D microenvironments perform more accurate in vivo mimicry than 2D models. Lee HJ et al. from Korea Research Institute of Bioscience and Biotechnology (KRIBB), prepared hybrid hydrogels of varying elasticity and compared them with a normal liver, to develop a more mature liver model that preserves liver properties in […]

26 febbraio 2018

Self-organized cerebral organoids predict effective drugs to combat Zika virus infection

The human cerebral cortex shows distinct structural and functional features: accordingly, great efforts have been concentrated on the development of human brain-like structures termed organoids. Watanabe and colleagues from David Geffen School of Medicine at UCLA have reported on Oct 2017 an optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia […]

19 febbraio 2018

Comprehensive high-throughput image analysis for therapeutic efficacy of architecturally complex heterotypic organoids

Bioengineered 3D organoids with heterotypic cellular composition are gaining interest as cancer models to be used to perform drug screening and therapy development. A team of scientists from Harvard Medical School and Massachusetts General Hospital (Boston, USA) describes a comprehensive image analysis procedure for structurally complex organotypic cultures (CALYPSO) applied to fluorescence-based assays to extract […]

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