3d biology

Goodbye flat biology

LIFE IS COMPLEX, DYNAMIC, PLASTIC: WHY STUDING FLAT 2D BIOLOGY?

3D, 360° DEGREE, TIME LAPSE

Sewing discrepancies between in vitro and in vivo models

Lack of reliable biological models of in vivo situation is a critical issue both for life science research and preclinical drug R&D.

3D tumor models more precisely recapitulate human solid tumor architecture cells was becoming and biology compared to 2D cell monolayers

VIDEO GALLERY

Preliminar results on CELLviewer platform are shown beside

  • Spheroid permeability to MitoGreen

    Sample:Amniotic Epithelial cell spheroid; Speed up: 40X
  • Fluorescent signal increase over time

    Sample: MCF-7 breast spheroid; Speed up: 360X
  • Spheroid viability

    Sample: Human Osteosarcoma spheroid; Speed up: 210X

    VIDEO GALLERY

    Preliminar results on CELLviewer platform are shown beside

    • Spheroid permeability to MitoGreen

      Sample:Amniotic Epithelial cell spheroid; Speed up: 40X
    • Fluorescent signal increase over time

      Sample: MCF-7 breast spheroid; Speed up: 360X
    • Spheroid viability

      Sample: Human Osteosarcoma spheroid; Speed up: 210X

      CANCER SPHEROIDS

      Source: Hirschhaeuser F. et al. Multicellular tumor spheroids: An underestimated tool is catching up again. J Biotechnol. 2010; 148(1):3-15

      LOTS OF INFORMATIONS TO BE EXTRACTED FROM

      Spheroids, 3D architectures of cancer cells, are found in cancer patients with variable micrometric sizes. They are composed of heterogeneous cells exposed to different environments cues.

      ACCURATE MODELS, RELIABLE RESULTS

      Spheroids show limited drug penetration since drugs are largely confined to the outer cell layers. They represent a more informative and predictive model for drug resistance than monolayer cultures.

      CANCER SPHEROIDS

      LOTS OF INFORMATIONS TO BE EXTRACTED FROM

      Spheroids, 3D architectures of cancer cells, are found in cancer patients with variable micrometric sizes. They are composed of heterogeneous cells exposed to different environments cues.

      ACCURATE MODELS, RELIABLE RESULTS

      Spheroids show limited drug penetration since drugs are largely confined to the outer cell layers. They represent a more informative and predictive model for drug resistance than monolayer cultures.

      TODAY’S CHALLENGE

      IMPLEMENT 3D MODELS IN DRUG R&D AND HTS SCREENING

      • Standardize spheroid culture method
      • Achieve spheroids population with homogeneous size and morphologies
      • Develop automated in vitro assays to reduce hands-on time and results variability
      • Define spheroids imaging analysis parameters to be physiologically relevant in pre- and clinical stage

      END-TO-END METHOD FOR SPHEROID CULTURE AND ANALYSIS

      CELLviewer isolates cancer spheroids and time-lapse monitor their dynamics and growth. Users can test drug toxicity and effiicacy on them and track cancer stem cell evolution in a in vivo-like environment.

      1. Cells seeding.

      Cells are plated in a 96 multiwell ultra low attachment plate in order to let them aggregate in spheroids.

      2. Cell culture

      Check spheroid formation with a microscope. Homogeneous size spheroid population is transferred in a 50 mL Falcon tube closed with a CELLviewer DOCK cap and loaded in CELLviewer.

      3. Sample loading

      Cells are withdrawn by microfluidics and collected in the microfluidic cartridge where a single spheroid is isolated in the analysis chamber.

      4. Drug dispensing

      Through automated liquid handling, drug solutions are dispensed to the sample in the analysis chamber to test compound efficacy and biological response

      5. Analysis

      Sample is kept in focus through Fluidic Adaptive Focusing and imaged in Bright-field and fluorescence channels. Images can be visualized in real time and acquisition parameters can be edited during the experiment.

      6. Data Handling

      Experiment data (images, videos, graphs, plots, etc.) can be real-time analyzed, saved on internal hard disk or external storage, exported and shared with cloud computing.
      WORKFLOW

      Registered Office

      Via Villa Clelia 62/D 40026 
      Imola (Bologna), Italy

      Laboratories

      Via Piero Gobetti 101 40129
      Bologna, Italy

      +39 051 639 9215
      info@celldynamics.it

      Registered Office

      Via Villa Clelia 62/D 40026
      Imola (Bologna), Italy

      Laboratories

      Via Piero Gobetti 101 40129
      Bologna, Italy

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      Contact us

      Contact us

      info@celldynamics.it


      +39 051 6399215

      Contact us

      info@celldynamics.it 
      +39 051 6399215

      Registered Office

      Via Villa Clelia 62/D 40026
      Imola (Bologna), Italy
      VAT: IT03544920360

      Laboratories

      Via Piero Gobetti 101 40129
      Bologna, Italy
      3d Biology for reliable Medicine
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