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19 aprile 2018

WORKSHOP: MAGNESIUM IN NEUROSCIENCE, PRECLINICAL AND CLINICAL FINDINGS

CELLviewer, a lab-on-a-chip technology for  and study of biological phenomena

Purpose:  Single-cell analysis is a fast growing field with a high impact in the research community owing to its numerous applications including cancer research, diagnostic, and drug discovery. In this context, microfluidics and lab-on-a-chip technology have emerged as the most promising avenue to address these challenges. Here, we used CELLviewer, an innovative laboratory bench prototype designed for continuous observation of floating cells, to monitor single cells and tumor spheroids.

Materials and methods: The device is a Lab-on-a-Chip platform based on the combination of optics, electronics and microfluidic technologies together with disposable modules, resulting in imaging tool. The sample is continuously monitored via an optical system, which detects its position, and a feedback mechanism within the optical and microfluidic systems adjusts cell position to the desired location. Moreover, intermittent light is used to reduce photodamage by prolonged exposure.

Results: By combining fluorescence time-lapse imaging, automated focal adjustment, culture media control technologies, CELLviewer allows us to perform uninterrupted observations of tumor single cells (HL60 and Jurkat) in conditioned medium, and of human osteosarcoma spheroids. Moreover, we are performing ongoing imaging tests of intracellular magnesium in cells and in spheroids of various origins by using a fluorescent dye (DCHQ5), which is able to assess total intracellular magnesium.

Conclusion: CELLviewer could be the proper tools to entrap floating organoids and 3D-cell aggregates for cell culture, imaging and study of biological phenomena. In particular, the application of this lab-on-a-chip technology in the study of magnesium distribution in 3D cellular models, could shed new lights in the comprehension of magnesium homeostasis in organoids.

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